Ketoprofen

Formulation

The table below describes the formulation of ketoprofen and the controls morphine sulfate and gabapentin for the experiments shown on this page.

Experiment(s)	Compound	Dose(s) mg/kg	Correction factor	Dose volume mL/kg	Route	Vehicle	Suspension / Solution
Pharmacokinetics	Ketoprofen	3, 6	1.00	2	PO	Saline	Suspension
Irwin, Rotarod	Ketoprofen	1, 3, 6, 10	1.00	5	PO	Saline	Solution
Plantar incision,	Ketoprofen	0.3, 1, 3, 6	1.00	5	PO	Saline	Solution
L5/L6 SNL	Ketoprofen	0.3, 1, 3, 6		5	PO		Solution
Plantar incision	Morphine	6	1.33	1	SC	Saline	Solution
L5/L6 SNL	Gabapentin	60	1.00	1	PO	Saline	Solution

L5/L6 SNL = L5/L6 spinal nerve ligation; PO = per os; SC = subcutaneous

Results

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Method: Pharmacokinetics

Ketoprofen concentrations were measured in serially collected plasma samples and terminally collected brain samples (shown on two graphs) from males and females treated with 3 or 6 mg/kg ketoprofen (PO) in a vehicle of saline. There were 4 rats/group for plasma and 3-4 rats/group for brain.

Method: Pharmacokinetics

A table shows pharmacokinetics parameters calculated for ketoprofen using non-compartmental analysis in male and female rats treated with ketoprofen (3 or 6 mg/kg, PO, vehicle of saline). The means for the 4 groups (males and females treated with 3 mg/kg ketoprofen and males and females treated with 6 mg/kg ketoprofen, respectively) are as follows. The highest concentration (Cmax) was 14.4, 25.5, 28.78, and 38.1 µM; the time at the maximum concentration (Tmax) was 3, 2, 1.13, and 1.75 hours; the half-life (T1/2) was 47.3, 9.07, 10.6, and 7.32 hours; area under the curve for time 0 to 24 hours was 13732, 15003, 20044, and 18655 minutes*µM; area under the curve for time 0 to infinity was 39010, 18891, 25101, and 21999 minutes*µM; the brain-to-plasma ratio at 1 hour was 0.01 for all groups; the brain-to-plasma ratio at 24 hours was 0 for 3 mg/kg groups and 0.01 for 6 mg/kg groups

Method: Modified Irwin functional observational battery

Two reviewers observed and scored 39 behaviors in a modified Irwin test. For each animal, the scores for behavioral observations from each reviewer are summed and then averaged for male and female rats (shown on two graphs). Responses are shown at the following time points: baseline (before treatment) and at 1, 2, 4, and 6 hours after treatment with vehicle (saline, delivered PO) or ketoprofen (1, 3, 6, or 10 mg/kg, delivered PO). There were 4 rats per group. Kruskal-Wallis tests were performed at individual timepoints, but no significant effects were found. Data were analyzed using GraphPad Prism version 10.0.2

Method: Modified Irwin functional observational battery

A heat map depicts the severity of the behaviors observed by two reviewers in a modified Irwin test at baseline and at 1, 2, 4, and 6 hours following the administration of vehicle (saline, delivered PO) or ketoprofen (1, 3, 6, or 10 mg/kg, delivered PO). There were 8 rats per group (4 male and 4 female). Severity score was calculated as the sum of scores given by the two reviewers for all animals at that dose and timepoint divided by the maximum possible score, transformed into a percentage. In summary, ketoprofen administration was well tolerated at all doses.

Method: Modified Irwin functional observational battery

Two graphs show the body temperature of male or female rats during the modified Irwin test. Responses are shown at the following timepoints: baseline (before treatment) and at 1 and 6 hours after treatment with vehicle (saline, delivered PO) or ketoprofen (1, 3, 6, or 10 mg/kg, delivered PO). There were 4 rats per group. A significant effect of time was found in a two-way repeated measures ANOVA for females. Dunnett’s tests found a significant decrease in body temperature relative to baseline at 6 hours post-treatment in females (p<0.0001). Data were analyzed using GraphPad Prism version 10.0.2.

Method: Rat Rotarod

Two graphs show the latency for male or female rats to fall off a rotarod apparatus. Responses are shown at the following time points: baseline (before treatment) and at 1, 2, 4, and 6 hours after treatment with vehicle (saline, delivered PO) or ketoprofen (1, 3, 6, or 10 mg/kg, delivered PO). There were 10 rats per group. No significant effects were found in a two-way repeated measures ANOVA for either sex. Data were analyzed using GraphPad Prism version 10.0.2

Model: Plantar Incision Model in Rat (Brennan Model)
Endpoint: Rat Hind Paw Mechanical Allodynia Behavior (von Frey Filaments) Method

Two graphs show ipsilateral hind paw mechanical allodynia behavior assessed with von Frey filaments in male or female rats that have undergone plantar incision surgery. Responses are shown at the following time points: baseline (before surgery), prior to treatment at 1 day after surgery, and at 1, 2, 4, and 6 hours after treatment at 1 day after surgery. The treatments are vehicle (saline, delivered PO), ketoprofen (0.3, 1, 3, or 6 mg/kg, delivered PO), or morphine (6 mg/kg, delivered SC). There were 10 rats per group. A significant interaction of time x treatment was found in a two-way repeated measures ANOVA for each sex. Bonferroni’s tests found significant increases in withdrawal thresholds relative to vehicle in males at 1, 2, and 4 hours post-treatment with morphine (p<0.0001, 0.0001, 0.001, respectively). Bonferroni’s tests found significant increases in withdrawal thresholds relative to vehicle in females at 1 and 2 hours post-treatment with morphine (p<0.0001, 0.01, respectively).

Model: Plantar Incision Model in Rat (Brennan Model)
Endpoint: Guarding Behavior

Two graphs show ipsilateral hind paw guarding behavior evaluated in male or female rats that have undergone plantar incision surgery. Responses are shown at the following time points: baseline (before surgery), prior to treatment at 1 day after surgery, and at 1, 2, 4, and 6 hours after treatment at 1 day after surgery. The treatments are vehicle (saline, delivered PO), ketoprofen (0.3, 1, 3, or 6 mg/kg, delivered PO), or morphine (6 mg/kg, delivered SC). There were 10 rats per group. A significant interaction of time x treatment was found in a two-way repeated measures ANOVA for each sex. Dunnett’s tests found significant decreases in guarding scores relative to vehicle in males at 1 hour post-treatment with 0.3 mg/kg ketoprofen (p<0.01), 1 mg/kg ketoprofen (p<0.05), 3 mg/kg ketoprofen (p<0.01), 6 mg/kg ketoprofen (p<0.01), and morphine (p<0.0001); at 2 hours post-treatment with 0.3 mg/kg ketoprofen (p<0.01), 1 mg/kg ketoprofen (p<0.01), 3 mg/kg ketoprofen (p<0.0001), 6 mg/kg ketoprofen (p<0.0001), and morphine (p<0.0001); at 4 hours post-treatment with 0.3 mg/kg ketoprofen (p<0.01), 1 mg/kg ketoprofen (p<0.01), 3 mg/kg ketoprofen (p<0.0001), and 6 mg/kg ketoprofen (p<0.0001); and at 6 hours post-treatment with 1 mg/kg ketoprofen (p<0.05), 3 mg/kg ketoprofen (p<0.001), and 6 mg/kg ketoprofen (p<0.01). Bonferroni’s tests found significant decreases in guarding scores relative to vehicle in females at 1 hour post-treatment with 0.3 mg/kg ketoprofen (p<0.05), 3 mg/kg ketoprofen (p<0.0001), 6 mg/kg ketoprofen (p<0.001), and morphine (p<0.0001); at 2 hours post-treatment with 0.3 mg/kg ketoprofen (p<0.001), 3 mg/kg ketoprofen (p<0.0001), 6 mg/kg ketoprofen (p<0.0001), and morphine (p<0.0001); at 4 hours post-treatment with post-treatment with 0.3 mg/kg ketoprofen (p<0.001), 1 mg/kg ketoprofen (p<0.05), 3 mg/kg ketoprofen (p<0.0001), and 6 mg/kg ketoprofen (p<0.0001); and at 6 hours post-treatment with post-treatment with 0.3 mg/kg ketoprofen (p<0.01), 1 mg/kg ketoprofen (p<0.01), 3 mg/kg ketoprofen (p<0.001), and 6 mg/kg ketoprofen (p<0.0001).

Model: L5/L6 Spinal Nerve Ligation Model
Endpoint: Rat Hind Paw Mechanical Allodynia Behavior (von Frey Filaments) Method

Model: L5/L6 Spinal Nerve Ligation Model
Endpoint: Rat Hind Paw Cold Allodynia (Acetone Evaporation) Test

Two graphs show ipsilateral hind paw cold allodynia behavior assessed with 5 trials of the acetone evaporation test in male or female rats that have undergone L5/L6 spinal nerve ligation surgery. Responses are shown at the following time points: baseline (before surgery), prior to treatment at 3 weeks after surgery, and at 2 and 4 hours after treatment at 3 weeks after surgery. The treatments are vehicle (saline, delivered PO), ketoprofen (0.3, 1, 3, or 6 mg/kg, delivered PO), or the positive control gabapentin (60 mg/kg, delivered PO). There were 10 rats per group. A significant interaction of time x treatment was found in a two-way repeated measures ANOVA for each sex. Dunnett’s tests found significant decreases in acetone responses relative to vehicle in males at 2 hours post-treatment with gabapentin (p<0.05). Dunnett’s tests found significant decreases in acetone responses relative to vehicle in females at 2 hours post-treatment with gabapentin (p<0.05).

This work was conducted by PsychoGenics Inc. (Paramus, NJ) in collaboration with PSPP, NINDS, NIH under contract # 75N95019D00026. Prescribing information for clinically used controls can be found at labels.fda.gov.

Ketoprofen

Formulation

Results

Pharmacokinetics: Concentration in Plasma and Brain

Pharmacokinetics: Calculated Parameters

Irwin: Total Observations

Irwin: Behavioral Severity Scores

Irwin: Body Temperature

Rotarod

Plantar Incision: von Frey

Plantar Incision: Guarding Behavior

L5/L6 Spinal Nerve Ligation: von Frey

L5/L6 Spinal Nerve Ligation: Acetone