RAT DYNAMIC WEIGHT BEARING MEASUREMENT METHOD
Studies are conducted in adult male and female Sprague Dawley rats (Envigo) in an AAALAC-accredited facility with approval from an Institutional Animal Care and Use Committee, and implemented in accordance with the Guide for the Care and Use of Laboratory Animals 8th Edition (National Research Council, 2011) with standards set by the National Institutes of Health.
A change in normal weight bearing of the paws in rodents is a translatable measure of limb pain and or joint pain/instability. This test, devoid of evoked stimulation or restraint, measures the changes in postural equilibrium in rodents by assessing their weight distribution on each of their 4 paws (Philpott et al., 2017).
Rats are placed in the Dynamic Weight Bearing Instrument 2.0 (Bioseb). The acquisition procedure starts after a rat is placed in the chamber (Figure 1). Following a 10 s latency, data are captured in real-time over a period of 180 s. Using the Dynamic Weight Bearing 2.0 software (Bioseb), the following data are summarized for each individual rat capturing: hind paw weight bearing (g), hind paw surface area on sensor pad (mm2), and hind paw time on surface pad (s).
This work was conducted by PsychoGenics Inc. (Paramus, NJ) in collaboration with PSPP, NINDS, NIH under contract # 75N95019D00026